A Novel Human Adipocyte-Derived Basement Membrane for Tissue Engineering Applications

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dc.contributor.advisor Nagrath, Deepak
dc.creator Damm, Aaron 2012-09-06T00:08:12Z 2012-09-06T00:08:15Z 2012-09-06T00:08:12Z 2012-09-06T00:08:15Z 2012-05 2012-09-05 May 2012
dc.description.abstract Tissue engineering strategies have traditionally focused on the use of synthetic polymers as support scaffolds for cell growth. Recently, strategies have shifted towards a natural biologically derived scaffold, with the main focus on decellularized organs. Here, we report the development and engineering of a scaffold naturally secreted by human preadipocytes during differentiation. During this differentiation process, the preadipocytes remodel the extracellular matrix by releasing new extracellular proteins. Finally, we investigated the viability of the new basement membrane as a scaffold for tissue engineering using human pancreatic islets, and as a scaffold for soft tissue repair. After identifying the original scaffold material, we sought to improve the yield of material, treating the cell as a bioreactor, through various nutritional and cytokine stimuli. The results suggest that adipocytes can be used as bioreactors to produce a designer-specified engineered human extracellular matrix scaffold for specific tissue engineering applications.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subject Adipocytes
Extracellular Matrix
Growth Factor
Natural Biomaterials
dc.title A Novel Human Adipocyte-Derived Basement Membrane for Tissue Engineering Applications
dc.contributor.committeeMember Zygourakis, Kyriacos
dc.contributor.committeeMember Gonzalez, Ramon
dc.contributor.committeeMember Sabek, Omaima 2012-09-06T00:08:16Z
dc.identifier.slug 123456789/ETD-2012-05-89
dc.type.genre Thesis
dc.type.material Text Chemical and Biomolecular Engineering Engineering Rice University Masters Master of Science
dc.identifier.citation Damm, Aaron. (2012) "A Novel Human Adipocyte-Derived Basement Membrane for Tissue Engineering Applications." Masters Thesis, Rice University.

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