Show simple item record

dc.contributor.advisor Bartel, Bonnie
dc.creatorStoddard, Jerrad
dc.date.accessioned 2012-09-05T23:55:39Z
dc.date.accessioned 2012-09-05T23:56:36Z
dc.date.available 2013-09-05T05:10:05Z
dc.date.created 2012-05
dc.date.issued 2012-09-05
dc.date.submitted May 2012
dc.identifier.citation Stoddard, Jerrad. "Elucidating the Roles of PEX19 and Prenylation in Arabidopsis Peroxisomes." (2012) Master’s Thesis, Rice University. https://hdl.handle.net/1911/64615.
dc.identifier.urihttps://hdl.handle.net/1911/64615
dc.description.abstract Peroxisomes are organelles originating from the endoplasmic reticulum. Peroxisome biogenesis requires multiple peroxins, including PEX19, a prenylated protein that helps deliver peroxisomal membrane proteins in yeast and mammals. Arabidopsis thaliana PEX19 is encoded by two isogenes, PEX19A and PEX19B. I demonstrate that pex19A and pex19B insertional mutants lack obvious abberant physiological phenotypes. I provide evidence that pex19A pex19B double mutants are inviable, that PEX19B is more abundant than PEX19A in young seedlings, that Arabidopsis PEX19 is farnesylated in vivo, and that YFP-PEX19 predominantly associates with what appears to be a subcellular membrane regardless of its prenylation state. I show that farnesyltransferase mutants apparently contain only non-prenylated PEX19 and lack phenotypes that would indicate inefficient peroxisome activity. My analysis of PEX19 suggests that PEX19 prenylation is dispensable for peroxisome biogenesis, and has generated tools for future studies of the earliest steps in peroxisome biogenesis in plants.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subjectAuxin
Peroxin
Peroxisomes
Arabidopsis
dc.title Elucidating the Roles of PEX19 and Prenylation in Arabidopsis Peroxisomes
dc.contributor.committeeMember Shamoo, Yousif
dc.contributor.committeeMember Farach-Carson, Cindy
dc.contributor.committeeMember Braam, Janet
dc.contributor.committeeMember Rudgers, Jennifer A.
dc.date.updated 2012-09-05T23:56:36Z
dc.identifier.slug 123456789/ETD-2012-05-58
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Biochemistry and Cell Biology
thesis.degree.discipline Natural Sciences
thesis.degree.grantor Rice University
thesis.degree.level Masters
thesis.degree.name Master of Arts
dc.embargo.terms 2013-09-05T05:00:00Z


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record