Development of Image Mapping Spectrometer (IMS) for hyperspectral microscopy

Abstract

A snapshot hyperspectral imaging device - Image Mapping Spectrometer (IMS) - has been developed for hyperspectral microscopy. The IMS replaces the camera in a digital imaging system, allowing one to add high speed snapshot spectrum acquisition capability to a variety of imaging modalities, e.g. microscopy, endoscopy, macroscopic imaging, to maximize the collection speed. The IMS described in this thesis is a parallel acquisition instrument that captures a hyperspectral datacube without scanning. It also allows" full" light throughput across the whole spectral collection range due to its snapshot operating format. The developments of three generations of the IMS (low sampling prototype, high sampling IMS, ruggedized IMS)have been reported in this thesis. Two illumination methods (filterless full-spectrum and structured illumination) are also developed for the IMS to achieve specific imaging aims. To show the application of the IMS in realtime hyperspectral imaging of live cells, the Calcium and a FRET-based biosensor are monitored simultaneously in ,B-cell dynamics. The imaging results demonstrate that the IMS has become an important spectral imaging modality in the biological imaging applications.