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dc.contributor.advisor Tkaczyk, Tomasz S.
dc.creatorGao, Liang
dc.date.accessioned 2011-07-25T02:07:20Z
dc.date.available 2011-07-25T02:07:20Z
dc.date.issued 2010
dc.identifier.urihttps://hdl.handle.net/1911/62191
dc.description.abstract An image mapping spectrometer (IMS) for microscopy applications is presented. Its principle is based on the redirecting of image zones by specially organized thin mirrors within a custom fabricated component termed an image mapper. The demonstrated prototype can simultaneously acquire a 140nm spectral range within its 2D field of view from a single image. The spectral resolution of the system is 5.6nm. The FOV and spatial resolution of the IMS depend on the selected microscope objective and for the results presented is 45x45mu 2 and 0.45mum respectively. The system requires no scanning and minimal post data processing. In addition, the reflective nature of the image mapper and use of prisms for spectral dispersion make the system light efficient. Both of the above features are highly valuable for real time fluorescent-spectral imaging in biological and diagnostic applications.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subjectOptics
dc.title Development of image mapping spectrometer (IMS) for hyperspectral fluorescence microscopy
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Physics
thesis.degree.discipline Natural Sciences
thesis.degree.grantor Rice University
thesis.degree.level Masters
thesis.degree.name Master of Science
dc.identifier.citation Gao, Liang. "Development of image mapping spectrometer (IMS) for hyperspectral fluorescence microscopy." (2010) Master’s Thesis, Rice University. https://hdl.handle.net/1911/62191.


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