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dc.contributor.advisor Alvarez, Pedro J.
dc.creatorDominguez-Faus, Rosa
dc.date.accessioned 2009-06-03T21:07:43Z
dc.date.available 2009-06-03T21:07:43Z
dc.date.issued 2007
dc.identifier.urihttp://hdl.handle.net/1911/20501
dc.description.abstract Aquifer flow-through columns were operated for 12 weeks to evaluate the benefits of oxygen addition on the biodegradation of a Chlroboenzene (CB) DNAPL source zone. Quantitative PCR was used to measure total bacteria (16S rDNA) and several aromatic oxygenase genes. CB removal was faster in the oxygen-amended columns compared to a control column, and qPCR showed that whereas the biphenyl and toluene dioxygenase biomarkers were most abundant, increases in the biomarker concentration for the phenol hydroxylase gene reflected best the higher CB removal due to aerobic biostimulation. DGGE analyses of the soil amended with oxygen revealed the dominant presence of Rhodococcus erythropolis (89% sequence similarity), which belongs to a genus known for its ability to degrade many priority pollutants, including CBs.
dc.format.extent 42 p.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subjectEnvironmental science
Environmental engineering
dc.title Source zone bioremediation of chlorobenzene DNAPLs: Performance assessment using real time quantitative polymerase chain reaction
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Environmental Science and Engineering
thesis.degree.discipline Engineering
thesis.degree.grantor Rice University
thesis.degree.level Masters
thesis.degree.name Master of Science
dc.identifier.citation Dominguez-Faus, Rosa. "Source zone bioremediation of chlorobenzene DNAPLs: Performance assessment using real time quantitative polymerase chain reaction." (2007) Master’s Thesis, Rice University. http://hdl.handle.net/1911/20501.


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