Inverse model for the extraction of biological parameters from ovarian tissue fluorescence spectra and multivariate classifiers for tissue diagnosis
Drezek, Rebekah A.
Master of Science
We present an inverse model to decompose bulk fluorescence spectra and extract tissue biological parameters. By deconvolving the effects of absorption and scattering from measured spectra, we are able to extract the intrinsic contributions from cellular and stromal fluorophores. Ovarian fluorescence, acquired ex-vivo immediately upon removal from patients, are analyzed using this model. We test the validity of the inverse model, and show that it has the ability to improve our understanding of the biological changes that cause the observed differences in the fluorescence spectra. The outputs of the model are used to develop classifiers for tissue diagnosis. Classifiers based on PLS selected features are also developed. An accuracy of more than 93% for discrimination between normal and cancerous ovarian tissues is achieved.
Biomedical engineering; Biophysics