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dc.contributor.advisor Hughes, Joseph B.
dc.creatorWatrous, Mary Margaret
dc.date.accessioned 2009-06-04T08:02:04Z
dc.date.available 2009-06-04T08:02:04Z
dc.date.issued 2003
dc.identifier.urihttp://hdl.handle.net/1911/17632
dc.description.abstract Unique genetic modifications to C. acetobutylicum altered the level of hydrogenase expression, allowing study of the resulting effects on the 2,4,6-trinitrotoluene (TNT) reduction rates to be possible. A strain designed to over-express the hydrogenase gene resulted in maintained TNT reduction during late growth phases when it is not typically observed. Strains exhibiting under-expression of hydrogenase produced slower TNT rates of reduction correlating to the expected inhibition of each strain type. Hydrogenase activity, measured by hydrogen production, in Clostridium acetobutylicum correlates strongly (R2 = 0.89) to TNT reduction rates. Indications suggested that hydrogenase potentially played an integral role in catalysis of TNT transformation by reducing its nitro substituents to the corresponding hydroxylamines. A mechanistic pathway is proposed by which this transformation takes place and may enhance the understanding of commonly found hydrogenases in other microorganisms and their ability to transform nitroaromatic compounds.
dc.format.extent 94 p.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subjectMicrobiology
Biochemistry
Environmental engineering
dc.title 2,4,6-trinitrotoluene reduction by hydrogenase in Clostridium acetobutylicum
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Chemistry
thesis.degree.discipline Natural Sciences
thesis.degree.grantor Rice University
thesis.degree.level Masters
thesis.degree.name Master of Science
dc.identifier.citation Watrous, Mary Margaret. "2,4,6-trinitrotoluene reduction by hydrogenase in Clostridium acetobutylicum." (2003) Master’s Thesis, Rice University. http://hdl.handle.net/1911/17632.


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