Metabolism of 2,4,6-trinitrotoluene by Clostridium acetobutylicum: Pathway identification and lab-scale evaluation of contaminated soil bioremediation
Richardson, Adam David
Master of Science
Studies are presented investigating the metabolism of 2,4,6-trinitrotoluene (TNT) by Clostridium acetobutylicum. When incubated with a batch culture of acetogenic Clostridium acetobutylicum, TNT was reduced through 4-hydroxylamino-2,6-dinitrotoluene (and to a lesser extent 2-hydroxylamino-4,6-dinitrotoluene) to 2,4-dihydroxylamino-6-nitrotoluene. The intermediate 2,4-dihydroxylamino-6-nitrotoluene then underwent a microbially catalyzed Bamberger rearrangement to form 4-amino-6-hydroxylamino-4-methyl-2-nitrophenol. When incubated with TNT-contaminated soil, C. acetobutylicum was able to transform TNT to 2,4-dihydroxylamino-6-nitrotoluene and beyond. Additionally, aerobic soil bacteria indigenous to TNT contaminated soil were able to mineralize a fraction (approximately 9% to 10%) of the products created by the reduction of TNT by C. acetobutylicum.
Microbiology; Environmental science; Environmental engineering