Catharanthus roseus as a model system for the study of the phytoremediation of TNT
Lauritzen, John Robert, III
Master of Science
C. roseus hairy roots cultures were employed to prove the ability of plants to take up and transform TNT in the absence of microbes. Mass balances performed with 14C-TNT showed that the TNT was being taken up and transformed and not mineralized. The transformation pathway was seen to be at least initially reductive. However, in cases where no TNT was detectable at experiment's end, standards could only account for 5% of the final products. Pseudo-first order reaction kinetics were observed for low initial concentrations of TNT in both stationary and exponential phase hairy root cultures. Kinetic constants ranged from 0.0103 to 0.0161 L/g-day for stationary phase experiments performed with 15 to 35 mg/L initial TNT concentrations. Kinetic constants of 0.0075 to 0.0156 L/g-day were observed for experiments performed on exponential growth phase hairy roots with an initial TNT concentration of 20 to 40 mg/L. Toxic effects were seen at all phases of growth and all concentrations measured.
Agriculture; Plant Culture; Chemical engineering; Environmental science