Fabrication of pliable polymer scaffolds for tissue engineering and particulate effects on osteoblast function
Wake, Mary Conley
Master of Science
Pliable, biodegradable scaffolds with poly(lactic-co-glycolic acid) (PLGA) and poly(ethylene glycol) (PEG) blends have been fabricated using a solvent-casting and particulate-leaching technique. This study investigated the effects of different processing parameters, PLGA copolymer ratio, the PLGA/PEG blend ratio, the initial salt weight fraction, and the salt particle size, on scaffold pliability and pore morphology. A wide range of shear moduli, porosities, and median pore diameters was able to be achieved. Effects of biodegradable particles of poly(L-lactic acid) (PLLA) and PLGA on rat marrow stromal cells in vitro have also been investigated. This study examined the effects of three particle parameters, concentration, polymer molecular weight, and PLGA copolymer ratio, on osteoblast proliferation and function. The most significant trend observed was due to increasing the concentration of particles, resulting in decreased proliferation and function of osteoblasts throughout the 28 day study.
Biomedical engineering; Chemical engineering