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dc.creatorMILLER, LARRY ROBERT
dc.date.accessioned 2007-05-09T19:25:12Z
dc.date.available 2007-05-09T19:25:12Z
dc.date.issued 1980
dc.identifier.urihttps://hdl.handle.net/1911/15565
dc.description.abstract 5(alpha)-Cholest-8(14)-en-3(beta)-ol-15-one at 10('-4) M has been shown to inhibit the conversion of acetate, but not mevalonate, to nonsaponifiable lipids and digitoninprecipitable sterols by 10,000 x g supernatant preparations from rat liver homogenates. In addition, a variety of structurally related compounds were tested in this system. The direct addition of 5(alpha)-cholest-8(14)-en-3(beta)-ol-15-one to various cell-free preparations had no effect on the individual enzymes catalyzing the conversion of acetate to mevalonate. The administration of dietary 5(alpha)-cholest-8(14)-en-3(beta)-ol-15-one (0.1%) to male rats for eight days caused a significant decrease in serum cholesterol levels and an increase in the hepatic activities of acetoacetyl-CoA thiolase, HMG-CoA synthase, and HMG-CoA reductase. 14(alpha)-Ethyl-5(alpha)-cholest-7-en-3(beta),15-diol when administered by daily subcutaneous injections in triolein to male rats maintained on a Cholesterol-Free Test Diet has been shown to cause a 23 percent reduction in serum cholesterol levels after 13 days. A variety of oxygenated sterols containing a 15(alpha)-hydroxyl group were found to inhibit the conversion of acetate and mevalonate into digitonin-precipitable sterols by 10,000 x g supernatant preparations from rat liver homogenates. The structural parameters important in this inhibitory effect have been investigated. Cycloheximide at 10('-3) M has been found to inhibit the synthesis of digitonin-precipitable sterols from acetate, but not mevalonate, in 10,000 x g supernatant fractions of rat liver homogenates. This effect was not due to an inhibition of protein synthesis. The deprivation of food for 48 hours has been shown to cause a reduction in the activities of acetoacetyl-CoA thiolase, HMG-CoA synthase, and HMG-CoA reductase in rat liver.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subjectBiochemistry
dc.title STUDIES RELATIVE TO THE MECHANISM OF ACTION OF 15-OXYGENATED STEROLS
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Chemistry
thesis.degree.discipline Natural Sciences
thesis.degree.grantor Rice University
thesis.degree.level Doctoral
thesis.degree.name Doctor of Philosophy
dc.identifier.citation MILLER, LARRY ROBERT. "STUDIES RELATIVE TO THE MECHANISM OF ACTION OF 15-OXYGENATED STEROLS." (1980) Diss., Rice University. https://hdl.handle.net/1911/15565.


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