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dc.contributor.advisor Hughes, Joseph B.
dc.creatorKhan, Tariq A.
dc.date.accessioned 2009-06-03T23:56:25Z
dc.date.available 2009-06-03T23:56:25Z
dc.date.issued 1996
dc.identifier.urihttps://hdl.handle.net/1911/14073
dc.description.abstract In this work, rapid TNT ($>$150 mg/L) transformation by Clostridium acetobutylicum via a reductive pathway to monoaminonitrotoluenes, diaminonitrotoluenes, and on to undetectable end products was observed. The maximum velocities were greatest for TNT reduction, followed by 2A46DNT, 4A26DNT, and 24DA6NT. Initial reduction was preferred at the para position. The measured TNT transformation rates were considerably higher than those observed by others. The transformation of hydroxylamino intermediates was rate limiting. Rates and extents of transformation of TNT and its metabolites were affected by the organism's growth phase. When TNT was added in the early acidogenic phase, rapid transformation to undetectable compounds occurred. When added at the end of exponential growth, TNT and its intermediates were recalcitrant. The decline in transformation was putatively due to ferrodoxin and electron transfer inactivity. The results show potential for this organism to remediate TNT contamination, as in an ex situ bioslurry reactor amended with soluble glucose (sugar).
dc.format.extent 120 p.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subjectEnvironmental science
Chemical engineering
Microbiology
Biology
Engineering
dc.title Anaerobic transformation of 2,4,6-trinitrotoluene by Clostridium acetobutylicum
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Biology
thesis.degree.discipline Natural Sciences
thesis.degree.grantor Rice University
thesis.degree.level Masters
thesis.degree.name Master of Science
dc.identifier.citation Khan, Tariq A.. "Anaerobic transformation of 2,4,6-trinitrotoluene by Clostridium acetobutylicum." (1996) Master’s Thesis, Rice University. https://hdl.handle.net/1911/14073.


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