Effects of hydrocarbon sorption on biodegradation, emulsification capacity and microbial diversity of a mixed microbial population
Pointer, Judy Hill
Bedient, Philip B.
Master of Science
The presence of an adsorbent resin in broth/resin slurries exerted a selection pressure on a population of microorganisms isolated from gasoline contaminated soil. The resin acted as a non-degradable matrix to sorb and entrain the gasoline constituents and was used as a substitute for a homogeneous aquifer matrix of packed sand. When the resin and light non-aqueous phase liquids (LNAPLs) were present as the carbon source, two species, Acinetobacter lwoffi and Pseudomonas fluorescens, predominated over three other species in the original population. Concomitant with the diversity changes, emulsion production and degradation rates of low solubility compounds increased. At least two biosurfactants were produced, an extracellular surface tension reducer and an extracellular emulsifier. Only the emulsifier was associated with increased degradation and cell growth.
Environmental science; Microbiology; Civil engineering