Effect of novel antithrombotic agents on platelet aggregation under flow conditions
McIntire, Larry V.
Master of Science
Epi-fluorescence video microscopy and digital image processing were used to evaluate the effects of three novel anti-platelet agents namely GT-12/I.2HBr (GT-12/I), A4.2HBr and IC, a series of compounds containing the $-$3-carboxylic acid piperidine moiety as their functional group, on platelet aggregation. GT-12/I was evaluated at a concentration of 100$\mu$M so that it could be used as a reference compound against which the effects of A4.2HBr and IC could be compared. Results show that at this concentration GT-12/I inhibited platelet accumulation by 59.0 $\pm$ 3.8% whereas A4.2HBr, the more hydrophobic molecule, inhibited aggregation by 73.5 $\pm$ 1.9% at the same concentration. This confirms earlier predictions that the optimization of hydrophobicity within a compound maximizes its inhibitory potential. Stereochemistry, however, seems to dominate over all other effects as it was observed that IC, an enantiomer of GT-12/I, drastically reduced platelet aggregation by 84.8 $\pm$ 2.8%. (Abstract shortened with permission of author.)
Biomedical engineering; Chemical engineering