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dc.contributor.advisor Matthews, Kathleen S.
dc.creatorTaphouse, Carl Richard
dc.date.accessioned 2009-06-04T00:11:27Z
dc.date.available 2009-06-04T00:11:27Z
dc.date.issued 1992
dc.identifier.urihttps://hdl.handle.net/1911/13601
dc.description.abstract A density sensing factor has been isolated from Dictyostelium discoideum and is known as conditioned media factor (CMF). CMF has been shown to cause starved vegetative Dictyostelium cells to differentiate into one of two cell types, stalk or spore cells. One interesting aspect of CMF concerns its release: CMF mRNA is present in vegetative Dictyostelium cells, yet secretion of the CMF protein only occurs during early and late development. My research reveals evidence that CMF is sequestered in the plasma membrane of vegetative cells. Further experiments show that CMF anti-sense transformants are motile but do not form cell aggregates. I suggest that CMF may have a role in chemotaxis which could involve interactions with components of the cytoskeleton. I have identified a few cytoskeletal components in vegetative and developing Dictyostelium cells which may provide useful information concerning the developmental process.
dc.format.extent 65 p.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subjectCell biology
Molecular biology
dc.title Density sensing in Dictyostelium discoideum: Evidence for the sequestering of a density sensing factor in the plasma membrane and a role for this factor in cell aggregation
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Biology
thesis.degree.discipline Natural Sciences
thesis.degree.grantor Rice University
thesis.degree.level Masters
thesis.degree.name Master of Science
dc.identifier.citation Taphouse, Carl Richard. "Density sensing in Dictyostelium discoideum: Evidence for the sequestering of a density sensing factor in the plasma membrane and a role for this factor in cell aggregation." (1992) Master’s Thesis, Rice University. https://hdl.handle.net/1911/13601.


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