Characterization of T-lymphocyte-target cell adhesion using a parallel plate flow chamber
Hubbard, Brent Bryan
Glacken, W. Michael, Sc. D.
Master of Science
A 100 fold range of shear stress (.04-4 dynes/cm$\sp2$) was used to demonstrate that the level of shear used in conjugate enumeration assays affects the absolute magnitude of CTL-TC conjugates observed. Kinetic analysis of detachment reveals that there are weak and strong populations of conjugates. A reciprocal relationship may exist between the strength of the conjugates and the CTL's lytic ability. CTL's could reform conjugates and lyse appropriately after recovery of the lymphocytes from the flow assay. Specific and nonspecific CTL-TC conjugates increased with time of incubation 1.6 and 1.5 fold, respectively, when the preincubation period was increased from 5 to 10 minutes. Experiments performed at 37$\sp\circ$C showed that specific CTL-TC adhesions become stronger (1.5 fold) while nonspecific adhesions are not altered. Finally, use of mabs in the adhesion assay showed that specific adhesion could be blocked by mabs to lyt 2, but not T3, at.04 dynes/cm$\sp2$.
Chemical engineering; Immunology; Biomedical engineering