Now showing items 1-5 of 5
Cofactor engineering of intracellular CoA/acetyl-CoA and its subsequent effect on isoamyl acetate production in Escherichia coli
Traditional metabolic engineering focused on pathway manipulation strategies like amplification addition or deletion of pathway to manipulate fluxes. However, cofactors play an essential role in cellular metabolism and ...
Application of back-propagation neural networks to system identification and process control
Certain properties of the back-propagation neural network have been found to be potentially useful in structuring models for process control applications. The network's relative simplicity and its ability to learn by example ...
Biochemical and genetic engineering strategies to enhance recombinant protein production in Escherichia coli
Escherichia coli has been widely used to produce high-value recombinant proteins for years. Although high recombinant protein productivity can be attained, perhaps the most important goal for such processes, that of achieving ...
Metabolic engineering of cofactors (NADH/NAD+) in Escherichia coli
Metabolic engineering studies have generally focused on manipulating enzyme levels. However, cofactors play a major role in the production of fermentation products. This thesis provides the first systematic study of cofactor ...
A study of the fluid mechanics and the cultivation of mammalian cells in a magnetically stabilized fluidized bed bioreactor
The culture of anchorage dependent mammalian cells on microcarrier offers an attractive avenue for achieving high productivity of therapeutic and diagnostic proteins in bioreactors. Reducing production costs require high ...