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Cofactor engineering of intracellular CoA/acetyl-CoA and its subsequent effect on isoamyl acetate production in Escherichia coli
Traditional metabolic engineering focused on pathway manipulation strategies like amplification addition or deletion of pathway to manipulate fluxes. However, cofactors play an essential role in cellular metabolism and ...
Metabolic engineering of cofactors (NADH/NAD+) in Escherichia coli
Metabolic engineering studies have generally focused on manipulating enzyme levels. However, cofactors play a major role in the production of fermentation products. This thesis provides the first systematic study of cofactor ...