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dc.contributor.authorCorbett, Daniel C.
Fabyan, Wesley B.
Grigoryan, Bagrat
O'Connor, Colleen E.
Johansson, Fredrik
Batalov, Ivan
Regier, Mary C.
DeForest, Cole A.
Miller, Jordan S.
Stevens, Kelly R.
dc.date.accessioned 2020-11-06T02:02:48Z
dc.date.available 2020-11-06T02:02:48Z
dc.date.issued 2020
dc.identifier.citation Corbett, Daniel C., Fabyan, Wesley B., Grigoryan, Bagrat, et al.. "Thermofluidic heat exchangers for actuation of transcription in artificial tissues." Science Advances, 6, no. 40 (2020) AAAS: https://doi.org/10.1126/sciadv.abb9062.
dc.identifier.urihttps://hdl.handle.net/1911/109520
dc.description.abstract Spatial patterns of gene expression in living organisms orchestrate cell decisions in development, homeostasis, and disease. However, most methods for reconstructing gene patterning in 3D cell culture and artificial tissues are restricted by patterning depth and scale. We introduce a depth- and scale-flexible method to direct volumetric gene expression patterning in 3D artificial tissues, which we call “heat exchangers for actuation of transcription” (HEAT). This approach leverages fluid-based heat transfer from printed networks in the tissues to activate heat-inducible transgenes expressed by embedded cells. We show that gene expression patterning can be tuned both spatially and dynamically by varying channel network architecture, fluid temperature, fluid flow direction, and stimulation timing in a user-defined manner and maintained in vivo. We apply this approach to activate the 3D positional expression of Wnt ligands and Wnt/β-catenin pathway regulators, which are major regulators of development, homeostasis, regeneration, and cancer throughout the animal kingdom.
dc.language.iso eng
dc.publisher AAAS
dc.rights This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.title Thermofluidic heat exchangers for actuation of transcription in artificial tissues
dc.type Journal article
dc.citation.journalTitle Science Advances
dc.citation.volumeNumber 6
dc.citation.issueNumber 40
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1126/sciadv.abb9062
dc.type.publication publisher version
dc.citation.articleNumber eabb9062


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