Show simple item record

dc.contributor.authorYuan, Qing-Bin
Huang, Ya-Meng
Wu, Wen-Bin
Zuo, Pengxiao
Hu, Nan
Zhou, Yong-Zhang
Alvarez, Pedro J.J.
dc.date.accessioned 2019-08-28T16:10:12Z
dc.date.available 2019-08-28T16:10:12Z
dc.date.issued 2019
dc.identifier.citation Yuan, Qing-Bin, Huang, Ya-Meng, Wu, Wen-Bin, et al.. "Redistribution of intracellular and extracellular free & adsorbed antibiotic resistance genes through a wastewater treatment plant by an enhanced extracellular DNA extraction method with magnetic beads." Environment International, 131, (2019) Elsevier: https://doi.org/10.1016/j.envint.2019.104986.
dc.identifier.urihttps://hdl.handle.net/1911/107372
dc.description.abstract Due to the limitations of current extraction methods, extracellular DNA (eDNA) is rarely discerned from intracellular DNA (iDNA) despite having unique contributions to antibiotic resistance genes (ARGs) propagation. Furthermore, eDNA may be free (f-eDNA) or adsorbed to or suspended solids, including cells (a-eDNA), which affects ARG persistence and transmissivity. We developed a novel method using magnetic beads to separate iDNA, a-eDNA, and f-eDNA to assess how these physical states of ARGs change across a wastewater treatment plant. This method efficiently extracted eDNA (>85.3%) with higher recovery than current methods such as alcohol precipitation, CTAB-based extraction, and DNA extraction kits (<10%). Biological treatment and UV disinfection decreased the concentration of intracellular ARGs (iARGs) and adsorbed extracellular ARGs (a-eARGs), causing an increase of released free extracellular ARGs (f-eARGs). More ARGs were discharged through the wasted biosolids than in the effluent; iARGs and a-eARGs are prevalent in wasted biosolids ((73.9 ± 22.5) % and (23.4 ± 15.3) % of total ARGs respectively), while f-eARGs were prevalent in the effluent ((90.3 ± 16.5) %). Bacterial community analysis showed significant correlations between specific genera and ARGs (e.g., Aeromonas, Pseudomonas and Acinetobacter were strongly correlated with multidrug-resistance gene blaTEM). This treatment system decreased the discharge of iARGs to receiving environments, however, increased eARG concentrations were present in the effluent, which may contribute to the environmental resistome.
dc.language.iso eng
dc.publisher Elsevier
dc.rightsThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/).
dc.rights.urihttp://creativecommons.org/licenses/BY-NC-ND/4.0/
dc.title Redistribution of intracellular and extracellular free & adsorbed antibiotic resistance genes through a wastewater treatment plant by an enhanced extracellular DNA extraction method with magnetic beads
dc.type Journal article
dc.citation.journalTitle Environment International
dc.subject.keywordAntibiotic resistance genes
Bacterial community
DNA extraction
Extracellular DNA
Magnetic beads
Wastewater treatment facility
dc.citation.volumeNumber 131
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1016/j.envint.2019.104986
dc.identifier.pmid 31299601
dc.type.publication publisher version
dc.citation.articleNumber 104986


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record