Show simple item record

dc.contributor.authorRajan, Sriivatsan G.
Gallik, Kristin L.
Monaghan, James R.
Uribe, Rosa A.
Bronner, Marianne E.
Saxena, Ankur
dc.date.accessioned 2019-08-28T16:10:12Z
dc.date.available 2019-08-28T16:10:12Z
dc.date.issued 2018
dc.identifier.citation Rajan, Sriivatsan G., Gallik, Kristin L., Monaghan, James R., et al.. "Tracking neural crest cell cycle progressionᅠin vivo." genesis, 56, no. 6-7 (2018) Wiley: https://doi.org/10.1002/dvg.23214.
dc.identifier.urihttp://hdl.handle.net/1911/107370
dc.description.abstract Analysis of cell cycle entry/exit and progression can provide fundamental insights into stem cell propagation, maintenance, and differentiation. The neural crest is a unique stem cell population in vertebrate embryos that undergoes long-distance collective migration and differentiation into a wide variety of derivatives. Using traditional techniques such as immunohistochemistry to track cell cycle changes in such a dynamic population is challenging, as static time points provide an incomplete spatiotemporal picture. In contrast, the fluorescent, ubiquitination-based cell cycle indicator (Fucci) system provides in vivo readouts of cell cycle progression and has been previously adapted for use in zebrafish. The most commonly used Fucci systems are ubiquitously expressed, making tracking of a specific cell population challenging. Therefore, we generated a transgenic zebrafish line, Tg(-4.9sox10:mAG-gmnn(1/100)-2A-mCherry-cdt1(1/190)), in which the Fucci system is specifically expressed in delaminating and migrating neural crest cells. Here, we demonstrate validation of this new tool and its use in live high-resolution tracking of cell cycle progression in the neural crest and derivative populations.
dc.language.iso eng
dc.publisher Wiley
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.
dc.title Tracking neural crest cell cycle progressionᅠin vivo
dc.type Journal article
dc.citation.journalTitle genesis
dc.subject.keywordFucci
Sox10
zebrafish
dc.citation.volumeNumber 56
dc.citation.issueNumber 6-7
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1002/dvg.23214
dc.identifier.pmcid PMC6143351
dc.identifier.pmid 29956448
dc.type.publication post-print
dc.citation.articleNumber e23214


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record