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    Modularity and Hierarchy in CRISPR-Cas Target Recognition

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    Author
    Bonomo, Melia E.
    Date
    2018-04-04
    Advisor
    Deem, Michael W.
    Degree
    Master of Science
    Abstract
    Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) constitute a unique and highly adaptive immune system in prokaryotes. Sequences from invading mobile genetic elements, such as bacteriophage, are incorporated into the CRISPR locus to generate a heritable, immunological memory. We have reviewed a large body of CRISPR-Cas research to explore themes of co- evolutionary dynamics, horizontal gene transfer, specificity and cross-reactivity, cost and regulation, non-immunological CRISPR functions that boost host cell robust- ness, as well as applicable mechanisms for efficient and specific genetic engineering. We have also developed a model to show that the CRISPR-Cas machinery evolved with selection for modularity and hierarchy in order to effectively recognize invaders. Physical understanding of the CRISPR-Cas system, especially the specificity of target recognition, will advance uses in biotechnology.
    Keyword
    CRISPR-Cas; immune system; modeling; biotechnology
    Citation
    Bonomo, Melia E.. "Modularity and Hierarchy in CRISPR-Cas Target Recognition." (2018) Master’s Thesis, Rice University. https://hdl.handle.net/1911/105691.
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    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map