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dc.contributor.authorLu, Wei
Schafer, Nicholas P.
Wolynes, Peter G.
dc.date.accessioned 2019-01-09T17:21:15Z
dc.date.available 2019-01-09T17:21:15Z
dc.date.issued 2018
dc.identifier.citation Lu, Wei, Schafer, Nicholas P. and Wolynes, Peter G.. "Energy landscape underlying spontaneous insertion and folding of an alpha-helical transmembrane protein into a bilayer." Nature Communications, 9, (2018) Springer Nature: https://doi.org/10.1038/s41467-018-07320-9.
dc.identifier.urihttps://hdl.handle.net/1911/105032
dc.description.abstract Membrane protein folding mechanisms and rates are notoriously hard to determine. A recent force spectroscopy study of the folding of an α-helical membrane protein, GlpG, showed that the folded state has a very high kinetic stability and a relatively low thermodynamic stability. Here, we simulate the spontaneous insertion and folding of GlpG into a bilayer. An energy landscape analysis of the simulations suggests that GlpG folds via sequential insertion of helical hairpins. The rate-limiting step involves simultaneous insertion and folding of the final helical hairpin. The striking features of GlpG's experimentally measured landscape can therefore be explained by a partially inserted metastable state, which leads us to a reinterpretation of the rates measured by force spectroscopy. Our results are consistent with the helical hairpin hypothesis but call into question the two-stage model of membrane protein folding as a general description of folding mechanisms in the presence of bilayers.
dc.language.iso eng
dc.publisher Springer Nature
dc.rights This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.title Energy landscape underlying spontaneous insertion and folding of an alpha-helical transmembrane protein into a bilayer
dc.type Journal article
dc.citation.journalTitle Nature Communications
dc.citation.volumeNumber 9
dc.identifier.digital s41467-018-07320-9
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1038/s41467-018-07320-9
dc.identifier.pmcid PMC6251876
dc.identifier.pmid 30470737
dc.type.publication publisher version
dc.citation.articleNumber 4949


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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.