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dc.contributor.authorChen, Ye
Ho, Joanne M. L.
Shis, David L.
Gupta, Chinmaya
Long, James
Wagner, Daniel S.
Ott, William
Josić, Krešimir
Bennett, Matthew R.
dc.date.accessioned 2019-01-09T17:21:15Z
dc.date.available 2019-01-09T17:21:15Z
dc.date.issued 2018
dc.identifier.citation Chen, Ye, Ho, Joanne M. L., Shis, David L., et al.. "Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors." Nature Communications, 9, (2018) https://doi.org/10.1038/s41467-017-02473-5.
dc.identifier.urihttps://hdl.handle.net/1911/105031
dc.description.abstract One challenge for synthetic biologists is the predictable tuning of genetic circuit regulatory components to elicit desired outputs. Gene expression driven by ligand-inducible transcription factor systems must exhibit the correct ON and OFF characteristics: appropriate activation and leakiness in the presence and absence of inducer, respectively. However, the dynamic range of a promoter (i.e., absolute difference between ON and OFF states) is difficult to control. We report a method that tunes the dynamic range of ligand-inducible promoters to achieve desired ON and OFF characteristics. We build combinatorial sets of AraC-and LasR-regulated promoters containing -10 and -35 sites from synthetic and Escherichia coli promoters. Four sequence combinations with diverse dynamic ranges were chosen to build multi-input transcriptional logic gates regulated by two and three ligand-inducible transcription factors (LacI, TetR, AraC, XylS, RhlR, LasR, and LuxR). This work enables predictable control over the dynamic range of regulatory components.
dc.language.iso eng
dc.rights This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.title Tuning the dynamic range of bacterial promoters regulated by ligand-inducible transcription factors
dc.type Journal article
dc.citation.journalTitle Nature Communications
dc.citation.volumeNumber 9
dc.identifier.digital s41467-017-02473-5
dc.contributor.publisher Springer Nature
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.1038/s41467-017-02473-5
dc.identifier.pmcid PMC5754348
dc.identifier.pmid 29302024
dc.type.publication publisher version
dc.citation.articleNumber 64


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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.