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    Amino acid transport in Trypanosome gambiense

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    Author
    Hansen, Brian David
    Date
    1974
    Advisor
    Fisher, Frank M.||Read, Clark P.
    Degree
    Master of Arts
    Abstract
    Using C-labeled amino acids, absorption of L-lysine, L-arginine, L-glutamate, L-phenylalanine, L-methionine, L-threonine, L-alanine, and L-glycine were studied. All amino acids appeared to be absorbed by both simple diffusion and mediated systems. Inhibitions produced by analogues indicate 5 possible transport loci. Locus A may have 3 Bites, one which transports lysine and the remaining 2 binding glutamate and arginine. When occupied, the latter 2 appear to stimulate the transport of lysine. Methionine and phenylalanine were shown to bind nonproductively at the lysine transport site. Locus B transports threonine, glycine, and alanine with negligible interaction with the other amino acids studied. This locus is partially Na+-sensitive. Locus C transports glutamate primarily. Locus D has 2 transport sites, one for phenylalanine and one for methionine. Threonine shows slight interaction at this site. Locus E also has 2 transport sites, one for lysine and one for arginine. All 8 amino acids were tested for Na+ sensitivity, with only the amino acids entering at Locus B affected. Further evidence for Na+ sensitivity of Locus B was demonstrated in that the uptake of threonine, glycine, and alanine was ouabain-sensitive. The uptake of methionine and phenylalanine was measured in the presence of an amino acid mixture and the observed values agreed with those predicted by calculation according to the technique of Read et al. (1963).
    Citation
    Hansen, Brian David. "Amino acid transport in Trypanosome gambiense." (1974) Master’s Thesis, Rice University. http://hdl.handle.net/1911/104426.
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    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892