Effect of Haploid Syndrome abnormalities on germ cell number in Rana Pipiens
Stettner, Sandra Lorraine
Master of Arts
This investigation attempts to ascertain whether or not abnormal morphology in embryos of Rana pipiens, such as that seen in haploid animals, has an effect on the number of germ cells present on the gonadal ridge. The possible effects of anomalous morphology were excluded from any effects of ploidy by creating diploid heart-extirpated embryos which exhibit haploid-like symptoms. Counts of dissociated germ cells done at stage 25 through stage 25+3 showed, overall, no significant difference in germ cell numbers between haploids, diploids and heart-extirpated animals. Since embryos with abnormal morphology had similar germ cell numbers as normal animals, no effect on germ cell numbers can be attributed to the morphological anomalies of the haploid syndrome. No significant difference was detected by germ cell size comparisons between haploid, diploid and heart-extirpated animals. This finding is compared to cell size studies of haploids, diploids and triploids at other stages.