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dc.contributor.advisor Grande-Allen, K. Jane
dc.creatorSrinivas, Vittal
dc.date.accessioned 2018-12-03T18:33:10Z
dc.date.available 2018-12-03T18:33:10Z
dc.date.issued 2009
dc.identifier.urihttps://hdl.handle.net/1911/103727
dc.description.abstract The evolution of glycosaminoglycans (GAGs) in eukaryotic species has been one of great biological mysteries. This thesis investigates the composition, rate of degradation and solution characteristics of aggrecan proteoglycan derived GAGs through a variety of biochemical and biophysical means, thereby providing a window to understanding these complex biopolymers. Aim 1 covers the development and application a of novel tandem enzymatic strategy (lyase + hydrolase) to characterize the various sulfated products of GAG breakdown. Aim 2 applies strategies of analytical ultracentrifugation, crowding kinetics and a variety of biophysical methods to access GAG fine structure and molecular composition and to understand how this degradation may be modulated in vivo. Aim 3 follows directly from Aim 2 in assessing how bulk behavior of these biological polymers varies in a concentration dependent manner using rheology along with other crowders to understand scaling relationships. Overall, these aims provide a new viewpoint to understand, length, size, shape and compositional complexity of glycosaminoglycans as well as their specific degradation characteristics. This research will facilitate the development and application of biotherapeutic GAG derived polymers where changes in backbone adducts and stereochemistry could activate the colossal amount of potentially useful chemical compounds that are generally inert, dormant or only transiently active in the extracellular space.
dc.format.extent 315 pp
dc.language.iso eng
dc.subjectPolymer chemistry
Biomedical engineering
Applied sciences
Pure sciences
Biopolymers
Glycans
Glycosaminoglycan
dc.title Orthogonal approaches for studying glycosaminoglycan biopolymer
dc.identifier.digital 750848627
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Bioengineering
thesis.degree.discipline Engineering
thesis.degree.grantor Rice University
thesis.degree.level Doctoral
thesis.degree.name Doctor of Philosophy
dc.identifier.callno THESIS BIOENG. 2010 SRINIVAS
dc.identifier.citation Srinivas, Vittal. "Orthogonal approaches for studying glycosaminoglycan biopolymer." (2009) Diss., Rice University. https://hdl.handle.net/1911/103727.


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