Rice Univesrity Logo
    • FAQ
    • Deposit your work
    • Login
    View Item 
    •   Rice Scholarship Home
    • Rice University Graduate Electronic Theses and Dissertations
    • Rice University Electronic Theses and Dissertations
    • View Item
    •   Rice Scholarship Home
    • Rice University Graduate Electronic Theses and Dissertations
    • Rice University Electronic Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Orthogonal approaches for studying glycosaminoglycan biopolymer

    Thumbnail
    Name:
    750848627.pdf
    Size:
    13.00Mb
    Format:
    PDF
    View/Open
    Author
    Srinivas, Vittal
    Date
    2009
    Advisor
    Grande-Allen, K. Jane
    Degree
    Doctor of Philosophy
    Abstract
    The evolution of glycosaminoglycans (GAGs) in eukaryotic species has been one of great biological mysteries. This thesis investigates the composition, rate of degradation and solution characteristics of aggrecan proteoglycan derived GAGs through a variety of biochemical and biophysical means, thereby providing a window to understanding these complex biopolymers. Aim 1 covers the development and application a of novel tandem enzymatic strategy (lyase + hydrolase) to characterize the various sulfated products of GAG breakdown. Aim 2 applies strategies of analytical ultracentrifugation, crowding kinetics and a variety of biophysical methods to access GAG fine structure and molecular composition and to understand how this degradation may be modulated in vivo. Aim 3 follows directly from Aim 2 in assessing how bulk behavior of these biological polymers varies in a concentration dependent manner using rheology along with other crowders to understand scaling relationships. Overall, these aims provide a new viewpoint to understand, length, size, shape and compositional complexity of glycosaminoglycans as well as their specific degradation characteristics. This research will facilitate the development and application of biotherapeutic GAG derived polymers where changes in backbone adducts and stereochemistry could activate the colossal amount of potentially useful chemical compounds that are generally inert, dormant or only transiently active in the extracellular space.
    Keyword
    Polymer chemistry; Biomedical engineering; Applied sciences; Pure sciences; Biopolymers; More... Glycans; Glycosaminoglycan Less...
    Citation
    Srinivas, Vittal. "Orthogonal approaches for studying glycosaminoglycan biopolymer." (2009) Diss., Rice University. https://hdl.handle.net/1911/103727.
    Metadata
    Show full item record
    Collections
    • Rice University Electronic Theses and Dissertations [13405]

    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map

     

    Searching scope

    Browse

    Entire ArchiveCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsTypeThis CollectionBy Issue DateAuthorsTitlesSubjectsType

    My Account

    Login

    Statistics

    View Usage Statistics

    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map