Targeting A549 and MCF7 with small bivalent molecules coated on liposomes
Nguyen, Andrew To
Grande-Allen, K. Jane
Master of Science
Bilamellar invaginated vesicles (BIVs) are liposomal/nanopartical delivery vehicles that have the ability to penetrate tight barriers throughout the target organ including the interstitial pressure gradient in large solid tumors, and they have prolonged half-lives in vivo and stability in the circulation. However, there is currently a lack of available ligands that could target these BIVs to specific cell types. Bivalent small molecules (smaller than peptides) have been synthesized to mimic secondary protein structures found at hot-spots in protein-protein interactions. Therefore, they have the potential to target specific cells and avoid the immune system due to their small size. A library of these compounds has been screened using a novel high-throughput luciferase assay against MCF7 and A549 cells. There were 7 compounds that were found to have specifically targeted MCF7 cells, and 9 compounds that specifically targeted A549 cells in vitro.
Biomedical research; Oncology; Health and environmental sciences; Applied sciences