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dc.contributor.advisor Matthews, Kathleen S.
dc.creatorLiu, Ying
dc.date.accessioned 2018-12-03T18:32:14Z
dc.date.available 2018-12-03T18:32:14Z
dc.date.issued 2007
dc.identifier.urihttps://hdl.handle.net/1911/103652
dc.description.abstract Hox transcription factors direct differentiation in all tissue layers and many organ systems and therefore have highly specific tissue-dependent functions. The DNA-binding homeodomain of Hox proteins can bind a wide array of nucleotide sequences with similar high affinities, suggesting regions outside the homeodomain must impact DNA interaction. Utilizing a series of deletion mutants, we identified three binding affinity modulation regions outside the homeodomain. DNA binding is inhibited ∼2-fold by the YPWM motif and microexon regions (I1) and ∼40-fold by the large disordered I2 region. High affinity binding is partially restored by the N-terminal 174 residues (R region) in a length-dependent manner. Both I2 and R regions partially overlap the Ubx transcription activation domain, allowing communication between these functional systems. Evolutionary variations in the amino acid sequences of most of these regions potentially differentiate Ubx•DNA interactions.
dc.format.extent 261 pp
dc.language.iso eng
dc.subjectBiophysics
Biological sciences
DNA binding Hox proteins
Transcriptional regulation
Ultrabithorax
dc.title Exploration of DNA binding selectivity, transcriptional regulation, and evolutionary relationships of the Hox protein Ultrabithorax
dc.identifier.digital 304815410
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Biochemistry and Cell Biology
thesis.degree.discipline Natural Sciences
thesis.degree.grantor Rice University
thesis.degree.level Doctoral
thesis.degree.name Doctor of Philosophy
dc.identifier.callno THESIS BIOCHEM. 2008 LIU
dc.identifier.citation Liu, Ying. "Exploration of DNA binding selectivity, transcriptional regulation, and evolutionary relationships of the Hox protein Ultrabithorax." (2007) Diss., Rice University. https://hdl.handle.net/1911/103652.


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