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dc.contributor.advisor Gomer, Richard
dc.creatorChoe, Jonathan Myongwoo
dc.date.accessioned 2018-12-03T18:31:09Z
dc.date.available 2018-12-03T18:31:09Z
dc.date.issued 2008
dc.identifier.urihttps://hdl.handle.net/1911/103524
dc.description.abstract Dictyostelium cells secrete factors during growth and starvation. AprA (autocrine proliferation repressor), a 60 kDa protein secreted as part of a 150 kDa protein complex, functions as a cell proliferation repressor. In the present study, we expressed recombinant AprA (rAprA) in bacteria. We found that rAprA is bioactive as a proliferation repressor and that rAprA binds to live Dictyostelium cells. rAprA, when added to the growth medium of wild-type and aprA - cells, slows the proliferation of these cells; however, rAprA does not slow the proliferation of crlA - or cfaD - cells. CrlA is a putative receptor, and CfaD is a protein secreted by cells. These findings indicated that rAprA needs CrlA and CfaD to act as a proliferation repressor. However, cells lacking CrlA bound rAprA with high affinity, suggesting that CrlA may not be the receptor for AprA. Thus, AprA binds to cell surface receptors in a signal transduction pathway that involves CrlA and CfaD to negatively regulate cell proliferation.
dc.format.extent 45 pp
dc.language.iso eng
dc.subjectBiochemistry
Pure sciences
dc.title AprA: An autocrine, secreted factor that represses cell proliferation in Dictyostelium discoideum
dc.identifier.digital 304507169
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Biochemistry and Cell Biology
thesis.degree.discipline Natural Sciences
thesis.degree.grantor Rice University
thesis.degree.level Masters
thesis.degree.name Master of Arts
dc.identifier.callno THESIS BIOCHEM. 2008 CHOE
dc.identifier.citation Choe, Jonathan Myongwoo. "AprA: An autocrine, secreted factor that represses cell proliferation in Dictyostelium discoideum." (2008) Master’s Thesis, Rice University. https://hdl.handle.net/1911/103524.


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