Show simple item record

dc.contributor.authorZhou, Jin
Yang, Xiaoning
Liu, Wei
Wang, Chunlan
Shen, Yuan
Zhang, Fengzhi
Zhu, Huimin
Sun, Hongji
Chen, Jiayun
Lam, Johnny
Mikos, Antonios G.
Wang, Changyong
dc.date.accessioned 2018-09-26T14:52:44Z
dc.date.available 2018-09-26T14:52:44Z
dc.date.issued 2018
dc.identifier.citation Zhou, Jin, Yang, Xiaoning, Liu, Wei, et al.. "Injectable OPF/graphene oxide hydrogels provide mechanical support and enhance cell electrical signaling after implantation into myocardial infarct." Theranostics, 8, no. 12 (2018) Ivyspring: 3317-3330. https://doi.org/10.7150/thno.25504.
dc.identifier.urihttps://hdl.handle.net/1911/102719
dc.description.abstract After myocardial infarction (MI), the scar tissue contributes to ventricular dysfunction by electrically uncoupling viable cardiomyocytes in the infarct region. Injection of a conductive hydrogel could not only provide mechanical support to the infarcted region, but also synchronize contraction and restore ventricular function by electrically connecting isolated cardiomyocytes to intact tissue. Methods: We created a conductive hydrogel by introducing graphene oxide (GO) nanoparticles into oligo(poly(ethylene glycol) fumarate) (OPF) hydrogels. The hydrogels were characterized by AFM and electrochemistry workstation. A rat model of myocardial infarction was used to investigate the ability of OPF/GO to improve cardiac electrical propagation in the injured heart in vivo. Echocardiography (ECHO) was used to evaluate heart function 4 weeks after MI. Ca2+ imaging was used to visualize beating cardiomyocytes (CMs). Immunofluorescence staining was used to visualize the expression of cardiac-specific markers. Results: OPF/GO hydrogels had semiconductive properties that were lacking in pure OPF. In addition, the incorporation of GO into OPF hydrogels could improve cell attachment in vitro. Injection of OPF/GO 4 weeks after myocardial infarction in rats enhanced the Ca2+ signal conduction of cardiomyocytes in the infarcted region in comparison with PBS or OPF alone. Moreover, the injection of OPF/GO hydrogel into the infarct region enhanced the generation of cytoskeletal structure and intercalated disc assembly. Echocardiography analysis showed improvement in load-dependent ejection fraction/fractional shortening of heart function 4 weeks after injection. Conclusions: We prepared a conductive hydrogel (OPF/GO) that provide mechanical support and biological conduction in vitro and in vivo. We found that injected OPF/GO hydrogels can provide mechanical support and electric connection between healthy myocardium and the cardiomyocytes in the scar via activating the canonical Wnt signal pathway, thus upregulating the generation of Cx43 and gap junction associated proteins. Injection of OPF/GO hydrogel maintained better heart function after myocardial infarction than the injection of a nonconductive polymer.
dc.language.iso eng
dc.publisher Ivyspring
dc.rightsThis is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/).
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.title Injectable OPF/graphene oxide hydrogels provide mechanical support and enhance cell electrical signaling after implantation into myocardial infarct
dc.type Journal article
dc.citation.journalTitle Theranostics
dc.subject.keywordconduction
injectable biomaterials
myocardial infarction
remodeling
dc.citation.volumeNumber 8
dc.citation.issueNumber 12
dc.identifier.digital v08p3317
dc.type.dcmi Text
dc.identifier.doihttps://doi.org/10.7150/thno.25504
dc.identifier.pmcid PMC6010993
dc.identifier.pmid 29930732
dc.type.publication publisher version
dc.citation.firstpage 3317
dc.citation.lastpage 3330


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/).
Except where otherwise noted, this item's license is described as This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/).