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dc.contributor.authorCalderon, Christopher P.
Chen, Wei-Hung
Lin, Kuan-Jiuh
Harris, Nolan C.
Kiang, Ching-Hwa
dc.date.accessioned 2018-06-19T17:13:02Z
dc.date.available 2018-06-19T17:13:02Z
dc.date.issued 2008-09
dc.identifier.citation Calderon, Christopher P., Chen, Wei-Hung, Lin, Kuan-Jiuh, et al.. "Quantifying DNA Melting Transitions Using Single-Molecule Force Spectroscopy." (2008) https://hdl.handle.net/1911/102098.
dc.identifier.urihttps://hdl.handle.net/1911/102098
dc.description.abstract We stretched a DNA molecule using atomic force microscope and quantified the mechanical properties associated withᅠBandᅠSᅠforms of double-stranded DNA (dsDNA), molten DNA, and single-stranded DNA (ssDNA). We also fit overdamped diffusion models to the AFM time series and used these models to extract additional kinetic information about the system. Our analysis provides additional evidence supporting the view that S-DNA is a stable intermediate encountered during dsDNA melting by mechanical force. In addition, we demonstrated that the estimated diffusion models can detect dynamical signatures of conformational degrees of freedom not directly observed in experiments.
dc.format.extent 12 pp
dc.title Quantifying DNA Melting Transitions Using Single-Molecule Force Spectroscopy
dc.type Technical report
dc.date.note September 2008
dc.type.dcmi Text


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