Now showing items 1-4 of 4

    • A Rice CRISPy Treat: Improving CRISPR-Cas9 gene editing in the zebrafish to facilitate analysis of genes implicated in neural angiogenesis in an F0 screen 

      Clements, Thomas P (2018-04-16)
      Scientists are eager for novel mutants to study gene function, evolutionary relationships, and even perform drug screens. Zebrafish are a well-established model for scientific research (Kimmel et al., 1995) and have been a premiere model for both forward and reverse genetic research. However, each current method to produce novel gene knockouts (KO) ...
    • Expanding the Enzymatic Activity of the Programmable Endonuclease Cas9 

      Rivera Longsworth, Gia Francesca (2018-08-09)
      The CRISPR-Cas9 gene editing system has revolutionized our ability to make targeted mutations in a variety of organisms. In zebrafish, we are able to use microinjections of Cas9 protein or mRNA alongside guide RNAs (gRNA) to create targeted insertions and deletions (indels) at a frequency sufficient for recovery of loss of function alleles. Combined ...
    • Immunosuppressed Adult Zebrafish Model of Mucormycosis 

      Tatara, A.M.; Wurster, S.; Lockworth, C.R.; Albert, N.D.; Walsh, T.J.; (2018)
    • Tracking neural crest cell cycle progressionᅠin vivo 

      Rajan, Sriivatsan G.; Gallik, Kristin L.; Monaghan, James R.; Uribe, Rosa A.; Bronner, Marianne E.; (2018)
      Analysis of cell cycle entry/exit and progression can provide fundamental insights into stem cell propagation, maintenance, and differentiation. The neural crest is a unique stem cell population in vertebrate embryos that undergoes long-distance collective migration and differentiation into a wide variety of derivatives. Using traditional techniques ...