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    • Confirming putative variants at ≤ 5% allele frequency using allele enrichment and Sanger sequencing 

      Yan, Yan Helen; Chen, Sherry X.; Cheng, Lauren Y.; Rodriguez, Alyssa Y.; Tang, Rui; (2021)
      Whole exome sequencing (WES) is used to identify mutations in a patient’s tumor DNA that are predictive of tumor behavior, including the likelihood of response or resistance to cancer therapy. WES has a mutation limit of detection (LoD) at variant allele frequencies (VAF) of 5%. Putative mutations called at ≤ 5% VAF are frequently due to sequencing ...