| dc.contributor.advisor |
Wilson, Lon J. |
dc.creator |
Berger, Christopher Scott
|
| dc.date.accessioned |
2011-07-25T02:07:09Z |
| dc.date.available |
2011-07-25T02:07:09Z |
| dc.date.issued |
2010 |
| dc.identifier.uri |
http://hdl.handle.net/1911/62174
|
| dc.description.abstract |
For the first time, C60-monoclonal antibody (mAb) immunoconjugates have been determined to internalize into target cells using water-soluble Gd3+-ion-filled fullerenes (Gd C60(OH) x). Separate conjugations of Gd C60(OH)x with the antibody ZME-018 and a murine antibody mixture (MuIgG) took place in a 1:5 mAb:Gd 60 ratio. Quanitative analysis of the immunoconjugates was established using inductively-coupled plasma mass spectrometry (ICP-MS) and UV-Vis spectrometry (Gd C60+C60). Enzyme-linked immunosorbent assays (ELISA) show little change in the specific binding of the ZME-018 once conjugated.
Each immunoconjugate was exposed to two cancer cell lines, A375m (a ZME-018-specific line), and T24, a bladder carcinoma line. Internalization of the immunoconjugate was measured at various timepoints, after which the cells were harvested and digested with 25% HClO3 for Gd3+ analysis by ICP-MS.
ICP-MS results show immunoconjugate internalization peaked in the first hour of exposure of the cells with a large dropoff occuring in the subsequent hour. These are the first results demonstrating the practicality of a cancer therapy based on Fullerene Immunotherapy (FIT). |
| dc.format.mimetype |
application/pdf |
| dc.language.iso |
eng |
dc.subject |
Chemistry, General
Chemistry, Inorganic
|
| dc.title |
Gadolinium at carbon 60-(ZME-018) immunoconjugate targeting of A375 |
| dc.type.genre |
Thesis |
| dc.type.material |
Text |
| thesis.degree.discipline |
Chemistry |
| thesis.degree.grantor |
Rice University |
| thesis.degree.level |
Masters |
| thesis.degree.name |
Master of Arts |
| dc.identifier.citation |
Berger, Christopher Scott. "Gadolinium at carbon 60-(ZME-018) immunoconjugate targeting of A375." Masters Thesis, Rice University, ETD http://hdl.handle.net/1911/62174. |
