deposit_your_work

Gadolinium at carbon 60-(ZME-018) immunoconjugate targeting of A375

Files in this item

Files Size Format View
1486027.PDF 1.597Mb application/pdf Thumbnail

Show simple item record

Item Metadata

dc.contributor.advisor Wilson, Lon J.
dc.creator Berger, Christopher Scott
dc.date.accessioned 2011-07-25T02:07:09Z
dc.date.available 2011-07-25T02:07:09Z
dc.date.issued 2010
dc.identifier.uri http://hdl.handle.net/1911/62174
dc.description.abstract For the first time, C60-monoclonal antibody (mAb) immunoconjugates have been determined to internalize into target cells using water-soluble Gd3+-ion-filled fullerenes (Gd C60(OH) x). Separate conjugations of Gd C60(OH)x with the antibody ZME-018 and a murine antibody mixture (MuIgG) took place in a 1:5 mAb:Gd 60 ratio. Quanitative analysis of the immunoconjugates was established using inductively-coupled plasma mass spectrometry (ICP-MS) and UV-Vis spectrometry (Gd C60+C60). Enzyme-linked immunosorbent assays (ELISA) show little change in the specific binding of the ZME-018 once conjugated. Each immunoconjugate was exposed to two cancer cell lines, A375m (a ZME-018-specific line), and T24, a bladder carcinoma line. Internalization of the immunoconjugate was measured at various timepoints, after which the cells were harvested and digested with 25% HClO3 for Gd3+ analysis by ICP-MS. ICP-MS results show immunoconjugate internalization peaked in the first hour of exposure of the cells with a large dropoff occuring in the subsequent hour. These are the first results demonstrating the practicality of a cancer therapy based on Fullerene Immunotherapy (FIT).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subject Chemistry, General
Chemistry, Inorganic
dc.title Gadolinium at carbon 60-(ZME-018) immunoconjugate targeting of A375
dc.type.genre Thesis
dc.type.material Text
thesis.degree.discipline Chemistry
thesis.degree.grantor Rice University
thesis.degree.level Masters
thesis.degree.name Master of Arts
dc.identifier.citation Berger, Christopher Scott. (2010) "Gadolinium at carbon 60-(ZME-018) immunoconjugate targeting of A375." Masters Thesis, Rice University. http://hdl.handle.net/1911/62174.

This item appears in the following Collection(s)