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Gadolinium at carbon 60-(ZME-018) immunoconjugate targeting of A375

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dc.contributor.advisor Wilson, Lon J.
dc.creator Berger, Christopher Scott
dc.date.accessioned 2011-07-25T02:07:09Z
dc.date.available 2011-07-25T02:07:09Z
dc.date.issued 2010
dc.identifier.uri http://hdl.handle.net/1911/62174
dc.description.abstract For the first time, C60-monoclonal antibody (mAb) immunoconjugates have been determined to internalize into target cells using water-soluble Gd3+-ion-filled fullerenes (Gd C60(OH) x). Separate conjugations of Gd C60(OH)x with the antibody ZME-018 and a murine antibody mixture (MuIgG) took place in a 1:5 mAb:Gd 60 ratio. Quanitative analysis of the immunoconjugates was established using inductively-coupled plasma mass spectrometry (ICP-MS) and UV-Vis spectrometry (Gd C60+C60). Enzyme-linked immunosorbent assays (ELISA) show little change in the specific binding of the ZME-018 once conjugated. Each immunoconjugate was exposed to two cancer cell lines, A375m (a ZME-018-specific line), and T24, a bladder carcinoma line. Internalization of the immunoconjugate was measured at various timepoints, after which the cells were harvested and digested with 25% HClO3 for Gd3+ analysis by ICP-MS. ICP-MS results show immunoconjugate internalization peaked in the first hour of exposure of the cells with a large dropoff occuring in the subsequent hour. These are the first results demonstrating the practicality of a cancer therapy based on Fullerene Immunotherapy (FIT).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subject Chemistry, General
Chemistry, Inorganic
dc.title Gadolinium at carbon 60-(ZME-018) immunoconjugate targeting of A375
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Chemistry
thesis.degree.discipline Natural Sciences
thesis.degree.grantor Rice University
thesis.degree.level Masters
thesis.degree.name Master of Arts
dc.identifier.citation Berger, Christopher Scott. (2010) "Gadolinium at carbon 60-(ZME-018) immunoconjugate targeting of A375." Masters Thesis, Rice University. http://hdl.handle.net/1911/62174.

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